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The use of mRNA display to select high-affinity protein-binding peptides

We report the use of “mRNA display,” an in vitro selection technique, to identify peptide aptamers to a protein target. mRNA display allows for the preparation of polypeptide libraries with far greater complexity than is possible with phage display. Starting with a library of ≈10(13) random peptides...

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Detalhes bibliográficos
Main Authors: Wilson, David S., Keefe, Anthony D., Szostak, Jack W.
Formato: Artigo
Idioma:Inglês
Publicado em: The National Academy of Sciences 2001
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC31124/
https://ncbi.nlm.nih.gov/pubmed/11274392
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.061028198
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