Affinity selection of DNA-binding protein complexes using mRNA display

Comprehensive analysis of DNA–protein interactions is important for mapping transcriptional regulatory networks on a genome-wide level. Here we present a new application of mRNA display for in vitro selection of DNA-binding protein heterodimeric complexes. Under improved selection conditions using a...

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Autori principali: Tateyama, Seiji, Horisawa, Kenichi, Takashima, Hideaki, Miyamoto-Sato, Etsuko, Doi, Nobuhide, Yanagawa, Hiroshi
Natura: Artigo
Lingua:Inglês
Pubblicazione: Oxford University Press 2006
Soggetti:
Methods Online
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC1369289/
https://ncbi.nlm.nih.gov/pubmed/16478713
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gnj025
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