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Preparation and Fractionation of Xenopus laevis Egg Extracts

Crude and fractionated Xenopus egg extracts can be used to provide ingredients for reconstituting cellular processes for morphological and biochemical analysis. Egg lysis and differential centrifugation are used to prepare the crude extract which in turn in used to prepare fractionated extracts and...

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Détails bibliographiques
Auteurs principaux: K. Cross, Marie, Powers, Maureen
Format: Artigo
Langue:Inglês
Publié: MyJove Corporation 2008
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3110484/
https://ncbi.nlm.nih.gov/pubmed/19066500
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/891
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