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Mapping the orientation of nuclear pore proteins in living cells with polarized fluorescence microscopy

The nuclear pore complex (NPC) perforates the nuclear envelope to facilitate selective transport between nucleus and cytoplasm. The NPC is composed of multiple copies of ~30 different proteins, termed nucleoporins, whose arrangement within the NPC is a major unsolved puzzle in structural biology. Va...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Kampmann, Martin, Atkinson, Claire E., Mattheyses, Alexa L., Simon, Sanford M.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2011
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3109191/
https://ncbi.nlm.nih.gov/pubmed/21499242
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nsmb.2056
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