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Mapping the orientation of nuclear pore proteins in living cells with polarized fluorescence microscopy

The nuclear pore complex (NPC) perforates the nuclear envelope to facilitate selective transport between nucleus and cytoplasm. The NPC is composed of multiple copies of ~30 different proteins, termed nucleoporins, whose arrangement within the NPC is a major unsolved puzzle in structural biology. Va...

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Autores principales: Kampmann, Martin, Atkinson, Claire E., Mattheyses, Alexa L., Simon, Sanford M.
Formato: Artigo
Lenguaje:Inglês
Publicado: 2011
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC3109191/
https://ncbi.nlm.nih.gov/pubmed/21499242
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nsmb.2056
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