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Vector–Hexamer PCR Isolation of All Insert Ends from a YAC Contig of the Mouse Igh Locus

We have developed a simple PCR strategy, termed vector–hexamer PCR, that is unique in its ability to easily recover every insert end from large insert clones in YAC and BAC vectors. We used this method to amplify and isolate all insert ends from a YAC contig covering the mouse Igh locus. Seventy-sev...

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Bibliographic Details
Published in:Genome Res
Main Authors: Herring, Christopher D., Chevillard, Christophe, Johnston, Sean L., Wettstein, Peter J., Riblet, Roy
Format: Artigo
Language:Inglês
Published: Cold Spring Harbor Laboratory Press 1998
Subjects:
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC310737/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9647641/
https://ncbi.nlm.nih.govhttps://doi.org/10.1101/gr.8.6.673
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