Vector–Hexamer PCR Isolation of All Insert Ends from a YAC Contig of the Mouse Igh Locus
We have developed a simple PCR strategy, termed vector–hexamer PCR, that is unique in its ability to easily recover every insert end from large insert clones in YAC and BAC vectors. We used this method to amplify and isolate all insert ends from a YAC contig covering the mouse Igh locus. Seventy-sev...
Shranjeno v:
| izdano v: | Genome Res |
|---|---|
| Principais autores: | , , , , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
Cold Spring Harbor Laboratory Press
1998
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC310737/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9647641/ https://ncbi.nlm.nih.govhttps://doi.org/10.1101/gr.8.6.673 |
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