Vector–Hexamer PCR Isolation of All Insert Ends from a YAC Contig of the Mouse Igh Locus

We have developed a simple PCR strategy, termed vector–hexamer PCR, that is unique in its ability to easily recover every insert end from large insert clones in YAC and BAC vectors. We used this method to amplify and isolate all insert ends from a YAC contig covering the mouse Igh locus. Seventy-sev...

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Bibliografische Detailangaben

Veröffentlicht in:	Genome Res
Hauptverfasser:	Herring, Christopher D., Chevillard, Christophe, Johnston, Sean L., Wettstein, Peter J., Riblet, Roy
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Cold Spring Harbor Laboratory Press 1998
Schlagworte:	Genome Methods
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC310737/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9647641/ https://ncbi.nlm.nih.govhttps://doi.org/10.1101/gr.8.6.673
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