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Cloning-Free PCR-Based Allele Replacement Methods

Efficient homologous recombination permits the directed introduction of specific mutations into the yeast genome. Here we describe a cloning-free, PCR-based allele replacement method that simplifies allele transfer between yeast strains. The desired allele from one strain is amplified by PCR, along...

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Udgivet i:Genome Res
Principais autores: Erdeniz, Naz, Mortensen, Uffe H., Rothstein, Rodney
Format: Artigo
Sprog:Inglês
Udgivet: Cold Spring Harbor Laboratory Press 1997
Fag:
Letters
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC310678/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9414323/
https://ncbi.nlm.nih.govhttps://doi.org/10.1101/gr.7.12.1174
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