Cloning-Free PCR-Based Allele Replacement Methods
Efficient homologous recombination permits the directed introduction of specific mutations into the yeast genome. Here we describe a cloning-free, PCR-based allele replacement method that simplifies allele transfer between yeast strains. The desired allele from one strain is amplified by PCR, along...
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| Publicado en: | Genome Res |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Cold Spring Harbor Laboratory Press
1997
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC310678/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9414323/ https://ncbi.nlm.nih.govhttps://doi.org/10.1101/gr.7.12.1174 |
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