Cloning-Free PCR-Based Allele Replacement Methods
Efficient homologous recombination permits the directed introduction of specific mutations into the yeast genome. Here we describe a cloning-free, PCR-based allele replacement method that simplifies allele transfer between yeast strains. The desired allele from one strain is amplified by PCR, along...
Збережено в:
| Опубліковано в:: | Genome Res |
|---|---|
| Автори: | , , |
| Формат: | Artigo |
| Мова: | Inglês |
| Опубліковано: |
Cold Spring Harbor Laboratory Press
1997
|
| Предмети: | |
| Онлайн доступ: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC310678/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9414323/ https://ncbi.nlm.nih.govhttps://doi.org/10.1101/gr.7.12.1174 |
| Теги: |
Немає тегів, Будьте першим, хто поставить тег для цього запису!
|