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Design and specificity of hammerhead ribozymes against calretinin mRNA.

We obtained a partial sequence of mouse calretinin mRNA from cDNA clones, and designed hammerhead ribozymes to cleave positions within it. With a view to optimising hammerhead ribozymes for eliminating the mRNA in vivo, we varied the length and sequence of the three duplex 'arms' and measu...

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Main Authors: Ellis, J, Rogers, J
格式: Artigo
語言:Inglês
出版: 1993
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC310633/
https://ncbi.nlm.nih.gov/pubmed/8255773
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