High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome

African trypanosomes are major pathogens of humans and livestock and represent a model for studies of unusual protozoal biology. We describe a high-throughput phenotyping approach termed RNA interference (RNAi) target sequencing, or RIT-seq that, using Illumina sequencing, maps fitness-costs associa...

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Bibliographic Details
Main Authors: Alsford, Sam, Turner, Daniel J., Obado, Samson O., Sanchez-Flores, Alejandro, Glover, Lucy, Berriman, Matthew, Hertz-Fowler, Christiane, Horn, David
Format: Artigo
Language:Inglês
Published: Cold Spring Harbor Laboratory Press 2011
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Method
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3106324/
https://ncbi.nlm.nih.gov/pubmed/21363968
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.115089.110
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