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The stereochemical course of the first step of pre-mRNA splicing.
We have determined the effects on splicing of sulfur substitution of the non-bridging oxygens in the phosphodiester bond at the 5' splice site of a pre-mRNA intron. Pre-mRNAs containing stereochemically pure Rp and Sp phosphorothioate isomers were produced by ligation of a chemically synthesize...
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| Main Authors: | , |
|---|---|
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
1993
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| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC310585/ https://ncbi.nlm.nih.gov/pubmed/8265362 |
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