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Single base pair mutation analysis by PNA directed PCR clamping.

A novel method that allows direct analysis of single base mutation by the polymerase chain reaction (PCR) is described. The method utilizes the finding that PNAs (peptide nucleic acids) recognize and bind to their complementary nucleic acid sequences with higher thermal stability and specificity tha...

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Détails bibliographiques
Auteurs principaux: Orum, H, Nielsen, P E, Egholm, M, Berg, R H, Buchardt, O, Stanley, C
Format: Artigo
Langue:Inglês
Publié: 1993
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC310567/
https://ncbi.nlm.nih.gov/pubmed/8265345
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