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Single base pair mutation analysis by PNA directed PCR clamping.

A novel method that allows direct analysis of single base mutation by the polymerase chain reaction (PCR) is described. The method utilizes the finding that PNAs (peptide nucleic acids) recognize and bind to their complementary nucleic acid sequences with higher thermal stability and specificity tha...

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Detalhes bibliográficos
Main Authors: Orum, H, Nielsen, P E, Egholm, M, Berg, R H, Buchardt, O, Stanley, C
Formato: Artigo
Idioma:Inglês
Publicado em: 1993
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC310567/
https://ncbi.nlm.nih.gov/pubmed/8265345
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