Purification and properties of the MboII, a class-IIS restriction endonuclease.

After five purification steps a homogeneous preparation of endonuclease MboII was obtained, and several properties of the enzyme were determined. MboII is a monomer, with Mr under native and denaturing conditions being 47-49 x 10(3) Da. Endonuclease MboII is a basic protein (pI 8.3) which remains ac...

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Bibliografiske detaljer
Udgivet i:Nucleic Acids Res
Main Authors: Sektas, M, Kaczorowski, T, Podhajska, A J
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 1992
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC310404/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1741276/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/20.3.433
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