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Purification and properties of the MboII, a class-IIS restriction endonuclease.
After five purification steps a homogeneous preparation of endonuclease MboII was obtained, and several properties of the enzyme were determined. MboII is a monomer, with Mr under native and denaturing conditions being 47-49 x 10(3) Da. Endonuclease MboII is a basic protein (pI 8.3) which remains ac...
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| Autors principals: | , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
1992
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| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC310404/ https://ncbi.nlm.nih.gov/pubmed/1741276 |
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