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Purification and properties of the MboII, a class-IIS restriction endonuclease.
After five purification steps a homogeneous preparation of endonuclease MboII was obtained, and several properties of the enzyme were determined. MboII is a monomer, with Mr under native and denaturing conditions being 47-49 x 10(3) Da. Endonuclease MboII is a basic protein (pI 8.3) which remains ac...
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| Veröffentlicht in: | Nucleic Acids Res |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Oxford University Press
1992
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| Online Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC310404/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1741276/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/20.3.433 |
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