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Purification and properties of the MboII, a class-IIS restriction endonuclease.

After five purification steps a homogeneous preparation of endonuclease MboII was obtained, and several properties of the enzyme were determined. MboII is a monomer, with Mr under native and denaturing conditions being 47-49 x 10(3) Da. Endonuclease MboII is a basic protein (pI 8.3) which remains ac...

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Bibliografske podrobnosti
Main Authors: Sektas, M, Kaczorowski, T, Podhajska, A J
Format: Artigo
Jezik:Inglês
Izdano: 1992
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC310404/
https://ncbi.nlm.nih.gov/pubmed/1741276
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