Circular Permutation of Red Fluorescent Proteins

Circular permutation of fluorescent proteins provides a substrate for the design of molecular sensors. Here we describe a systematic exploration of permutation sites for mCherry and mKate using a tandem fusion template approach. Circular permutants retaining more than 60% (mCherry) and 90% (mKate) b...

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Main Authors: Shui, Bo, Wang, Qi, Lee, Frank, Byrnes, Laura J., Chudakov, Dmitry M., Lukyanov, Sergey A., Sondermann, Holger, Kotlikoff, Michael I.
Formato: Artigo
Idioma:Inglês
Publicado: Public Library of Science 2011
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Research Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC3103546/
https://ncbi.nlm.nih.gov/pubmed/21647365
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0020505
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