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Repair of a specific double-strand break generated within a mammalian chromosome by yeast endonuclease I-SceI.
We established a mouse Ltk- cell line that contains within its genome a herpes simplex virus thymidine kinase gene (tk) that had been disrupted by the insertion of the recognition sequence for yeast endonuclease I-SceI. The artificially introduced 18 bp I-SceI recognition sequence was likely a uniqu...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
1994
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| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC310129/ https://ncbi.nlm.nih.gov/pubmed/7838718 |
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