A new PCR based method for the generation of nested deletions.
We have developed a simple, PCR-based protocol, random primed/anchored-PCR (RPA-PCR), that allows the selective amplification and efficient cloning of segments that are adjacent to any known sequence. We demonstrate that RPA-PCR can be used to prepare a nested set of evenly spaced deletions suitable...
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| Vydáno v: | Nucleic Acids Res |
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| Hlavní autoři: | , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
Oxford University Press
1993
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| On-line přístup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC310022/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7690475/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/21.17.4143 |
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