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A new PCR based method for the generation of nested deletions.
We have developed a simple, PCR-based protocol, random primed/anchored-PCR (RPA-PCR), that allows the selective amplification and efficient cloning of segments that are adjacent to any known sequence. We demonstrate that RPA-PCR can be used to prepare a nested set of evenly spaced deletions suitable...
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| Hauptverfasser: | , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
1993
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| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC310022/ https://ncbi.nlm.nih.gov/pubmed/7690475 |
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