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Allelic discrimination by nick-translation PCR with fluorogenic probes.

Nick-translation PCR was performed with fluorogenic probes. Two probes were used: one complementary to a sequence containing the F508 codon of the normal human cystic fibrosis (CF) gene (wt DNA) and one complementary to a sequence containing the delta F508 three base pair deletion (mut DNA). Each pr...

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Detalles Bibliográficos
Autores principales: Lee, L G, Connell, C R, Bloch, W
Formato: Artigo
Lenguaje:Inglês
Publicado: 1993
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC309885/
https://ncbi.nlm.nih.gov/pubmed/8367293
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