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Quantitative, Fluorogenic Probe PCR Assay for Detection of Human Herpesvirus 8 DNA in Clinical Specimens

A quantitative, fluorescence-based PCR assay (TaqMan-based system) was developed for detection of human herpesvirus 8 (HHV-8) DNA in clinical specimens. Primers and probes chosen from each of five 10-kb segments from the unique region of the HHV-8 genome were evaluated for sensitivity with dilution...

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Detalhes bibliográficos
Main Authors: Stamey, Felicia R., Patel, Mitesh M., Holloway, Brian P., Pellett, Philip E.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2001
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC88385/
https://ncbi.nlm.nih.gov/pubmed/11574569
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.39.10.3537-3540.2001
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