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Allelic discrimination by nick-translation PCR with fluorogenic probes.
Nick-translation PCR was performed with fluorogenic probes. Two probes were used: one complementary to a sequence containing the F508 codon of the normal human cystic fibrosis (CF) gene (wt DNA) and one complementary to a sequence containing the delta F508 three base pair deletion (mut DNA). Each pr...
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| Main Authors: | , , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
1993
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| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC309885/ https://ncbi.nlm.nih.gov/pubmed/8367293 |
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