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Three-step PCR mutagenesis for 'linker scanning'.

'Linker scanning' has been used as an efficient method for systematically surveying a segment of DNA for functional elements by mutagenesis. A three-step PCR method was developed to simplify this process. In this method, a set of 'mutation primers' was made with 6 to 8 base subst...

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Détails bibliographiques
Auteurs principaux: Li, X M, Shapiro, L J
Format: Artigo
Langue:Inglês
Publié: 1993
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC309880/
https://ncbi.nlm.nih.gov/pubmed/8367290
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