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Three-step PCR mutagenesis for 'linker scanning'.

'Linker scanning' has been used as an efficient method for systematically surveying a segment of DNA for functional elements by mutagenesis. A three-step PCR method was developed to simplify this process. In this method, a set of 'mutation primers' was made with 6 to 8 base subst...

Täydet tiedot

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Bibliografiset tiedot
Julkaisussa:Nucleic Acids Res
Päätekijät: Li, X M, Shapiro, L J
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: Oxford University Press 1993
Linkit:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC309880/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8367290/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/21.16.3745
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