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Three-step PCR mutagenesis for 'linker scanning'.
'Linker scanning' has been used as an efficient method for systematically surveying a segment of DNA for functional elements by mutagenesis. A three-step PCR method was developed to simplify this process. In this method, a set of 'mutation primers' was made with 6 to 8 base subst...
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Main Authors: | , |
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
1993
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Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC309880/ https://ncbi.nlm.nih.gov/pubmed/8367290 |
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