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Rapid Screening of Complex DNA Samples by Single-Molecule Amplification and Sequencing
Microbial cloning makes Sanger sequencing of complex DNA samples possible but is labor intensive. We present a simple, rapid and robust method that enables laboratories without special equipment to perform single-molecule amplicon sequencing, although in a low-throughput manner, from sub-picogram qu...
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| Autori principali: | , , , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Public Library of Science
2011
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3098247/ https://ncbi.nlm.nih.gov/pubmed/21625543 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0019723 |
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