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Probing DNA-protein interactions in vitro with the CpG DNA methyltransferase.

A sensitive method was devised to monitor the in vitro binding of nuclear proteins from HeLa cells presumably to the major groove of DNA. Upon the incubation of DNA with nuclear extracts, the complexed DNA was incubated with the CpG DNA methyltransferase from Spiroplasma species. Subsequently, the D...

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Detalhes bibliográficos
Main Authors: Kochanek, S, Renz, D, Doerfler, W
Formato: Artigo
Idioma:Inglês
Publicado em: 1993
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC309529/
https://ncbi.nlm.nih.gov/pubmed/8506131
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