Differential cDNA cloning by enzymatic degrading subtraction (EDS).
We describe a new method, called enzymatic degrading subtraction (EDS), for the construction of subtractive libraries from PCR amplified cDNA. The novel features of this method are that i) the tester DNA is blocked by thionucleotide incorporation; ii) the rate of hybridization is accelerated by phen...
保存先:
| 出版年: | Nucleic Acids Res |
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| 主要な著者: | , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Oxford University Press
1994
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| オンライン･アクセス: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC308470/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7971268/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/22.21.4381 |
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