Differential cDNA cloning by enzymatic degrading subtraction (EDS).
We describe a new method, called enzymatic degrading subtraction (EDS), for the construction of subtractive libraries from PCR amplified cDNA. The novel features of this method are that i) the tester DNA is blocked by thionucleotide incorporation; ii) the rate of hybridization is accelerated by phen...
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| Publicado en: | Nucleic Acids Res |
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| Autores principales: | , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
Oxford University Press
1994
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| Acceso en línea: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC308470/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7971268/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/22.21.4381 |
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