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Polypyrimidine tract sequences direct selection of alternative branch sites and influence protein binding.
IVS1, an intron derived from the rat fibronectin gene, is spliced inefficiently in vitro, involving the use of three alternative branch sites. Mutation of one branch point site, BP3, so as to increase complementarity to U2 snRNA resulted in exclusive use of that site and improved splicing efficiency...
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| Главный автор: | |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
1994
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| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC308380/ https://ncbi.nlm.nih.gov/pubmed/7937104 |
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