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Measuring thermodynamic details of DNA hybridization using fluorescence

Modern real-time PCR systems make it easy to monitor fluorescence while temperature is varied for hundreds of samples in parallel, permitting high-throughput studies. We employed such system to investigate melting transitions of ordered nucleic acid structures into disordered random coils. Fluoresce...

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Bibliografski detalji
Glavni autori: You, Yong, Tataurov, Andrey V, Owczarzy, Richard
Format: Artigo
Jezik:Inglês
Izdano: Wiley Subscription Services, Inc., A Wiley Company 2011
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3082624/
https://ncbi.nlm.nih.gov/pubmed/21384337
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/bip.21615
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