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Thermal denaturation of double-stranded nucleic acids: prediction of temperatures critical for gradient gel electrophoresis and polymerase chain reaction.

A program is described which calculates the thermal stability and the denaturation behaviour of double-stranded DNAs and RNAs up to a length of 1000 base pairs. The algorithm is based on recursive generation of conditional and a priori probabilities for base stacking. Output of the program may be co...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Egile nagusia: Steger, G
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: 1994
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC308245/
https://ncbi.nlm.nih.gov/pubmed/8052531
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