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Thermal denaturation of double-stranded nucleic acids: prediction of temperatures critical for gradient gel electrophoresis and polymerase chain reaction.

A program is described which calculates the thermal stability and the denaturation behaviour of double-stranded DNAs and RNAs up to a length of 1000 base pairs. The algorithm is based on recursive generation of conditional and a priori probabilities for base stacking. Output of the program may be co...

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Detaylı Bibliyografya
Yayımlandı:Nucleic Acids Res
Yazar: Steger, G
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: Oxford University Press 1994
Online Erişim:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC308245/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8052531/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/22.14.2760
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