Thermal denaturation of double-stranded nucleic acids: prediction of temperatures critical for gradient gel electrophoresis and polymerase chain reaction.
A program is described which calculates the thermal stability and the denaturation behaviour of double-stranded DNAs and RNAs up to a length of 1000 base pairs. The algorithm is based on recursive generation of conditional and a priori probabilities for base stacking. Output of the program may be co...
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| Udgivet i: | Nucleic Acids Res |
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| Hovedforfatter: | |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Oxford University Press
1994
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| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC308245/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8052531/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/22.14.2760 |
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