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A novel fluorogenic substrate for ribonucleases. Synthesis and enzymatic characterization.
The synthesis and enzymatic characterization of DUPAAA, a novel fluorogenic substrate for RNases of the pancreatic type is described. It consists of the dinucleotide uridylyl-3',5'-deoxyadenosine to which a fluorophore, o-aminobenzoic acid, and a quencher, 2,4-dinitroaniline, have been att...
Bewaard in:
| Hoofdauteurs: | , , , , , |
|---|---|
| Formaat: | Artigo |
| Taal: | Inglês |
| Gepubliceerd in: |
1994
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| Online toegang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC308241/ https://ncbi.nlm.nih.gov/pubmed/8052528 |
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