Chimeric YACs were generated at unreduced rates in conditions that suppress coligation.

Chimerism is a major limitation of current YAC libraries. A method based on partially filled-in ends of restriction fragments was designed to avoid coligation as a possible source of chimeras. Model experiments using plasmid DNA as an insert showed that coligation was clearly avoided by this method....

Полное описание

Библиографические подробности

Опубликовано в::	Nucleic Acids Res
Главные авторы:	Wada, M, Abe, K, Okumura, K, Taguchi, H, Kohno, K, Imamoto, F, Schlessinger, D, Kuwano, M
Формат:	Artigo
Язык:	Inglês
Опубликовано:	Oxford University Press 1994
Online-ссылка:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC308044/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8202367/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/22.9.1651
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