Chimeric YACs were generated at unreduced rates in conditions that suppress coligation.
Chimerism is a major limitation of current YAC libraries. A method based on partially filled-in ends of restriction fragments was designed to avoid coligation as a possible source of chimeras. Model experiments using plasmid DNA as an insert showed that coligation was clearly avoided by this method....
Uloženo v:
| Vydáno v: | Nucleic Acids Res |
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| Hlavní autoři: | , , , , , , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
Oxford University Press
1994
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| On-line přístup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC308044/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8202367/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/22.9.1651 |
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