Učitavanje...
Chimeric YACs were generated at unreduced rates in conditions that suppress coligation.
Chimerism is a major limitation of current YAC libraries. A method based on partially filled-in ends of restriction fragments was designed to avoid coligation as a possible source of chimeras. Model experiments using plasmid DNA as an insert showed that coligation was clearly avoided by this method....
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| Glavni autori: | , , , , , , , |
|---|---|
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
1994
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| Online pristup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC308044/ https://ncbi.nlm.nih.gov/pubmed/8202367 |
| Oznake: |
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