Chimeric YACs were generated at unreduced rates in conditions that suppress coligation.
Chimerism is a major limitation of current YAC libraries. A method based on partially filled-in ends of restriction fragments was designed to avoid coligation as a possible source of chimeras. Model experiments using plasmid DNA as an insert showed that coligation was clearly avoided by this method....
Sábháilte in:
| Foilsithe in: | Nucleic Acids Res |
|---|---|
| Príomhchruthaitheoirí: | , , , , , , , |
| Formáid: | Artigo |
| Teanga: | Inglês |
| Foilsithe / Cruthaithe: |
Oxford University Press
1994
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| Rochtain ar líne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC308044/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8202367/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/22.9.1651 |
| Clibeanna: |
Níl clibeanna ann, Bí ar an gcéad duine le clib a chur leis an taifead seo!
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