Chimeric YACs were generated at unreduced rates in conditions that suppress coligation.

Chimerism is a major limitation of current YAC libraries. A method based on partially filled-in ends of restriction fragments was designed to avoid coligation as a possible source of chimeras. Model experiments using plasmid DNA as an insert showed that coligation was clearly avoided by this method....

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Publicat a:	Nucleic Acids Res
Autors principals:	Wada, M, Abe, K, Okumura, K, Taguchi, H, Kohno, K, Imamoto, F, Schlessinger, D, Kuwano, M
Format:	Artigo
Idioma:	Inglês
Publicat:	Oxford University Press 1994
Accés en línia:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC308044/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8202367/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/22.9.1651
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