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Expression, purification and preliminary crystallographic analysis of the recombinant β-­glucosidase (BglA) from the halothermophile Halothermothrix orenii

The β-glucosidase A gene (bglA) has been cloned from the halothermophilic bacterium Halothermothrix orenii and the recombinant enzyme (BglA; EC 3.2.1.21) was bacterially expressed, purified using metal ion-affinity chromatography and subsequently crystallized. Orthorhombic crystals were obtained tha...

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Bibliografski detalji
Glavni autori: Kori, Lokesh D., Hofmann, Andreas, Patel, Bharat K. C.
Format: Artigo
Jezik:Inglês
Izdano: International Union of Crystallography 2010
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3079986/
https://ncbi.nlm.nih.gov/pubmed/21206038
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309110046981
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