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Expression, purification and preliminary crystallographic analysis of sucrose phosphate synthase (SPS) from Halothermothrix orenii

This is the first report of the crystallization of a sucrose phosphate synthase (SPS; EC 2.4.1.14). It also constitutes the first study of a sucrose phosphate synthase from a non-photosynthetic thermohalophilic anaerobic bacterium, Halothermothrix orenii. The purified recombinant spsA protein has be...

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Detalhes bibliográficos
Main Authors: Huynh, Frederick, Tan, Tien-Chye, Swaminathan, Kunchithapadam, Patel, Bharat K. C.
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2004
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1952387/
https://ncbi.nlm.nih.gov/pubmed/16508108
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S174430910403091X
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