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Expression, purification, crystallization and preliminary X-ray analysis of wild-type and of an active-site mutant of glyceraldehyde-3-phosphate dehydrogenase from Campylobacter jejuni

The genome of the enteric pathogen Campylobacter jejuni encodes a single glyceraldehyde-3-phosphate dehydrogenase that can utilize either NADP(+) or NAD(+) as coenzymes for the oxidative phosphorylation of glyceraldehyde-3-­phosphate to 1,3-diphosphoglycerate. Here, the cloning, expression, purifica...

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Bibliografiske detaljer
Main Authors: Tourigny, David S., Elliott, Paul R., Edgell, Louise J., Hudson, Gregg M., Moody, Peter C. E.
Format: Artigo
Sprog:Inglês
Udgivet: International Union of Crystallography 2010
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3079976/
https://ncbi.nlm.nih.gov/pubmed/21206028
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309110044465
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