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Purification, crystallization and preliminary X-ray diffraction of the G3BP1 NTF2-like domain

The nuclear transport factor 2-like (NTF2-like) domain of human G3BP1 was subcloned, overexpressed in Escherichia coli and purified. Crystals were obtained using the hanging-drop vapour-diffusion method. Diffraction data were collected to 3.6 Å resolution using synchrotron radiation. The crystals be...

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Detalhes bibliográficos
Main Authors: Vognsen, Tina, Möller, Ingvar Rúnar, Kristensen, Ole
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3079970/
https://ncbi.nlm.nih.gov/pubmed/21206022
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309110042156
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