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Construction of a normalized cDNA library by introduction of a semi-solid mRNA-cDNA hybridization system.
We report a novel procedure to construct a normalized (equalized) cDNA library. By introduction of the highly efficient self-hybridization system between a whole mRNA population and their corresponding cDNA immobilized on latex beads, which involves relatively simple manipulations, we were able to g...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1994
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC307919/ https://ncbi.nlm.nih.gov/pubmed/8152931 |
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