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Construction of a normalized cDNA library by introduction of a semi-solid mRNA-cDNA hybridization system.

We report a novel procedure to construct a normalized (equalized) cDNA library. By introduction of the highly efficient self-hybridization system between a whole mRNA population and their corresponding cDNA immobilized on latex beads, which involves relatively simple manipulations, we were able to g...

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Detalles Bibliográficos
Main Authors: Sasaki, Y F, Ayusawa, D, Oishi, M
Formato: Artigo
Idioma:Inglês
Publicado: 1994
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC307919/
https://ncbi.nlm.nih.gov/pubmed/8152931
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