Allosteric Control of Ligand Binding Affinity Using Engineered Conformation-Specific Effector Proteins

We describe a phage display methodology to engineer synthetic antigen binders (sABs) that recognize either the apo- or the ligand-bound conformation of maltose binding protein (MBP). sABs that preferentially recognize the maltose-bound form of MBP act as positive allosteric effectors by significantl...

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Hauptverfasser: Rizk, Shahir S., Paduch, Marcin, Heithaus, John H., Duguid, Erica M., Sandstrom, Andrew, Kossiakoff, Anthony A.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2011
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3077571/
https://ncbi.nlm.nih.gov/pubmed/21378967
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nsmb.2002
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