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Characterization of the XRCC1-DNA ligase III complex in vitro and its absence from mutant hamster cells.

The human DNA repair protein XRCC1 was overexpressed as a histidine-tagged polypeptide (denoted XRCC1-His) in Escherichia coli and purified in milligram quantities by affinity chromatography. XRCC1-His complemented the mutant Chinese hamster ovary cell line EM9 when constitutively expressed from a p...

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Udgivet i:Nucleic Acids Res
Principais autores: Caldecott, K W, Tucker, J D, Stanker, L H, Thompson, L H
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 1995
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC307472/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8532526/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/23.23.4836
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