Characterization of the XRCC1-DNA ligase III complex in vitro and its absence from mutant hamster cells.
The human DNA repair protein XRCC1 was overexpressed as a histidine-tagged polypeptide (denoted XRCC1-His) in Escherichia coli and purified in milligram quantities by affinity chromatography. XRCC1-His complemented the mutant Chinese hamster ovary cell line EM9 when constitutively expressed from a p...
Spremljeno u:
| Izdano u: | Nucleic Acids Res |
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| Glavni autori: | , , , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
Oxford University Press
1995
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| Online pristup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC307472/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8532526/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/23.23.4836 |
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