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Non-radioactive detection of oligonucleotide probes by photochemical amplification of dioxetanes.

We describe a new method of non-radioactive labelling and detection of oligonucleotide probes. The approach is based on a simple chemical principle. Oligonucleotides labelled with methylene blue (a photosensitizer) are hybridized on a membrane to immobilized DNA target sequences. After hybridization...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Schubert, F, Knaf, A, Möller, U, Cech, D
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1995
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC307440/
https://ncbi.nlm.nih.gov/pubmed/8524657
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